Colorimetric vs enzymatic determination of serum phosphorus.
نویسندگان
چکیده
منابع مشابه
Determination of inorganic phosphorus in serum: Evaluation of three methods applied to the Technicon RA-1000 analyzer
We have evaluated three analytical methods for determining inorganic phosphorus in serum applied to the Technicon RA-I000 analyzer: a fully enzymatic colorimetric method based on the specific system purine nucleoside phosphorylase/xanthine oxidase coupled to an indicator colorimetric reaction similar to the Trinder reaction; a chemical method involving the direct UV measurement of the phosphomo...
متن کاملEnzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine.
The performance of an enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine is described. Phosphate ions react with inosine in the presence of purine nucleoside phosphorylase to form hypoxanthine; this is oxidized by xanthine oxidase to uric acid with production of hydrogen peroxide. The latter is determined with the aid of the chromogen system peroxidas...
متن کاملSerum profiles of calcium, phosphorus, magnesium, vitamin D and parathyroid hormone in Caspian horses during different seasons
Minerals play an essential role in the normal vital process.Calcium, phosphorus and magnesium are the most abundant minerals. Vitamin D and parathyroid hormone play a key role in regulation of their homeostasis as well. The aim of this study was to evaluate calcium, phosphorus, magnesium, vitamin D and parathyroid hormone on 30 Caspian horses of Guilan Province (south of the Caspian Sea) in two...
متن کاملNew Enzymatic Colorimetric Method for the Quantitative Determination of Phenylalanine in Dry-Blood Spots
Phenylketonuria (PKU) is an autosomal recessive disorder, which is characterized by severe mental retardation, microcephaly and seizures. The symptoms of this disease can be prevented if detected soon after birth. Therefore, blood Phenylalanine (Phe) measurement is essential for the early diagnosis, treatment and dietary monitoring of PKU patients. The goal of this research was to introduce a r...
متن کاملModification of Lofland's colorimetric semiautomated serum triglyceride determination, assessed by an enzymatic glycerol determination.
Erroneously high values for serum triglyceride levels obtained with the semiautomated method of Lofland were found to be due to contamination of the isopropanol extracts with glucose or other carbohydrate. Treatment of the isopropanol extracts with a mixture of copper sulfate and calcium hydroxide removed the contaminating glucose. Analysis of the glucose-free extracts by either the semiautomat...
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عنوان ژورنال:
- Clinical chemistry
دوره 30 10 شماره
صفحات -
تاریخ انتشار 1984